LinTT1 tumor penetrating peptide guided nanoparticles for improved imaging and therapy of glioblastoma
In a collaborative study “Peptide-guided nanoparticles for glioblastoma targeting” published in Journal of Controlled Release we evaluated a novel tumor penetrating peptide that targets cell surface p32, LinTT1 (AKRGARSTA), as a GBM targeting ligand for systemically-administered nanoparticles. LinTT1-functionalization increased tumor homing of iron oxide nanoworms (NWs) across a panel of five GBM models ranging from infiltratively-disseminating to angiogenic phenotypes. LinTT1-NWs homed to CD31-positive tumor blood vessels, including to transdifferentiated endothelial cells, and showed co-localization with tumor macrophages and lymphatic vessels. LinTT1-guided proapoptotic NWs exerted strong anti-glioma activity in two models of GBM, including doubling the lifespan of the mice in an aggressive orthotopic stem cell-like GBM that recapitulates the histological hallmarks of human GBM.
Lead authors of this exciting and potentially translationally relevant study were Pille Säälik and Prakash Lingasamy (both Laboratory of Cancer Biology). This work was performed in collaboration with the Department of Neurosurgery of Tartu University Clinics, team of Dr. Dinorah Friedmann-Morvinski at Tel Aviv University, and Prof. Rolf Bjerkvig at the University of Bergen.
Link to the abstract of the paper: https://www.ncbi.nlm.nih.gov/pubmed/31255690
Prof. Tambet Teesalu gave an inaugural lecture entitled “Nanomedicine: big opportunities in a small world”
Prof. Tambet Teesalu gave an inaugural lecture entitled “Nanomedicine: big opportunities in a small world” in the main hall of the University of Tartu on 14.05.2019.
This establishment of a professorship in nanomedicine at the University of Tartu underlines the importance that the University attributes to this exponentially growing interdisciplinary field of medicine.
Hedi Hunt successfully defended her Ph.D. thesis "Precision targeting of intraperitoneal tumors with peptide-guided nanocarriers" on April 18th, 2019. Her thesis dealt with the development of peptide-guided nanoparticles for therapy and imaging of peritoneal carcinomatosis. We wish Hedi good luck in her future career!
Image: Hedi Hunt and her opponent Professor Pirjo Laakkonen PhD of the University of Helsinki (Finland) during the thesis discussion.
We look for talented M.Sc. and PhD candidates (molecular medicine, cancer biology, drug delivery, nanomedicine) to join our lab in fall 2019.
For more information Click Here
Dr. Teesalu has given a series of invited talks on international conferences in Europe and in the US
Dr. Teesalu has given a series of invited talks on international conferences in Europe and in the US: The 4th Brain Tumors Conference 2018: From Biology to Therapy ( June 21-23, 2018, Warsaw, Poland), Frontiers in Delivery of Therapeutics (August 21-23 2018, Tartu, Estonia), 11th European and Global Summit for Clinical Nanomedicine, Targeted Delivery and Precision Medicine, „The Building Blocks to Personalized Medicine“ (September 2 ‐ 5, 2018, Basel Switzerland), Boulder Peptide Symposium (September 24-27, 2018, Boulder, USA), workshop on self assembly and hierarchical materials in biomedicine: drug delivery, tissue engineering, sensing and safety (October 8 – 10, 2018, San Sebastián, Spain). The mission of the talks was to increase the awareness of nanomedicine and drug delivery community on the tumor homing peptides developed at our laboratory and establish new collaborations.
Dr. Teesalu (left) with Dr. Sergio Moya (the organizer of the workshop on self assembly and hierarchical materials in biomedicine in San Sebastián, Spain
We have designed new nanoparticles that detect small breast tumors using the clinically used medical imaging modality Positron Emission Tomography (PET).
The particles are polymeric vesicles (polymersomes) made from biocompatible components polyethyleneglycol (PEG) and polycaprolactone (PCL). We found that functionalization with a tumor penetrating peptide discovered in our group ("LinTT1") boosted the accumulation of the particles in the tumor to allow sensitive detection of breast tumors modeled in mice. The polymersomes used in the study are versatile carriers that can be loaded with chemotherapeutic agents to make the drugs more selective and decrease side effects.
This work was performed in collaboration with the group of Dr. Sergio Moya at the Center of Cooperative Investigation in Biomaterials (CIC BiomaGUNE) in San Sebastian (Spain) and group of Prof. Twan Lammers of RWTH Aachen University, Aachen (Germany).
Image caption:PET images taken 48hours after intravenous injection of untargeted polymersomes and LinTT1-targeted polymersomes. The location of the tumors is marked with red arrow and both tumors had the same volume. The detection limit of the tumors using PET images was significantly lowered when using LinTT1 guided polymersomes.
Link to the paper: https://doi.org/10.18632/oncotarget.24588
The translational work at the Lab of Cancer Biology in 2017 was recognized by University of Tartu by awarding the head of the laboratory Tambet Teesalu the prize for research entrepreneurship.
Lorena, Hedi and Tambet from LCB published a review entitled „Peritoneal Carcinomatosis Targeting with Tumor Homing Peptides“ in Molecules (2018, 23(5), 1190; https://doi.org/10.3390/molecules23051190). The review summarizes progress from our and other research labs on affinity targeting of intraperitoneal anticancer compounds, imaging agents and nanoparticles with tumor-homing peptides. We review classes of tumor-homing peptides relevant for PC targeting, payloads for peptide-guided precision delivery, applications for targeted compounds, and the effects of nanoformulation of drugs and imaging agents on affinity-based tumor delivery.
Lab of cancer biology and other groups of the Deparment of Biomedicine (groups of Molecular Pathology, RNA Biology, and Human Genetics) had a day full of stimulating presentations and academic exchange at the beautiful Võrtsjärve Research Station/Lake Museum. Presenters from our laboratory were Anett Laarmann (Development of tumor penetrating antibodies), Anni Lepland (Therapeutic precision targeting of tumor-associated macrophages), Prakash Lingasamy (Dual-specific tumor targeting novel peptide for drug delivery), and Valeria Sidorenko (Smart nanoparticles for breast cancer detection and therapy).
Successful grant applications
Dr. Teesalu was awarded two prestigious grants:
(1) ~1M EUR Estonian Research Council grant to peptide-based develop transport systems to cross blood brain barrier
(2) 150.000 EUR European Research Council proof of concept grant to develop precision glioblastoma nanotherapies (the first ever ERC proof of concept grant to be awarded to an Estonian researcher).
In addition, Dr. Teesalu established a partnership in EUR 700.000 industrial collaboration project to develop affinity ligand-drug conjugates for precision cancer therapy with Toxinvent LLC (Tartu, Estonia).