Friday, 03 December 2021 15:35

New Tools for Streamlined In Vivo Homing Peptide Identification.

Põšnograjeva K, Pleiko K, Haugas M, Teesalu T. Methods Mol Biol. 2022;2383:385-412. doi: 10.1007/978-1-0716-1752-6_25. PMID: 34766303
In vivo peptide-phage display is an unbiased technique for mapping of the vascular diversity and identification of homing peptides. This chapter is intended to serve as a structured practical guide to execute in vivo T7 phage biopanning and data analysis experiments. We discuss experimental designs and protocols with emphasis on application of high-throughput sequencing-based technologies for streamlined in vivo biopanning and validation of homing peptides.