Linear TT1 peptide-coupled FAM-labeled iron oxide nanoparticles home to blood vessels of subcutaneous U87 tumor xenograft

Monday, 12 June 2017 16:03

Linear TT1 peptide-coupled FAM-labeled iron oxide nanoparticles (green) home to blood vessels (red) of subcutaneous U87 tumor xenograft. Cell nuclei are stained with DAPI (blue). Scale bar – 50 µm. Author – Pille Säälik PhD

Dark-field image of silver nanoparticles with RPARPAR-MMAE-AgNP internalized by PPC-1 prostate cancer cells

Friday, 12 May 2017 15:59

Dark-field image of silver nanoparticles with cytotoxic drug monomethyl auristatin E and RPARPAR peptide (RPARPAR-MMAE-AgNP) internalized by PPC-1 prostate cancer cells after 1 h incubation. Extracellular fraction of AgNPs was removed by etching. Scale bar: 20 μm; dotted lines outline cells. Author: Allan Tobi MSc.

PET-CT image of breast tumor bearing mouse i.v. injected with iRGD targeted nanoparticles

Wednesday, 19 April 2017 17:09

PET-CT scan was acquired 24 hours after sample administration. The radiolabeled iRGD-nanoparticles accumulates in breast tumor (white arrow). Authors: Lorena Simón Gracia and Pablo Scodeller

FAM-UNO homes to TIE2+ tumor macrophages

Sunday, 19 March 2017 17:06

Mouse model of triple negative breast cancer, 4T1. Microscope: Olympus FV1200MPE-BX61WI confocal microscope at 20X magnification Green: peptide, Red: TIE2, blue: cell nuclei. Intravenous injection. Author: Pablo Scodeller

Circular structure of blood vessels homing FAM-labeled nanoparticles in P3-stem like glioblastoma

Monday, 13 February 2017 18:02

Circular structure of blood vessels homing FAM-labeled nanoparticles in P3-stem like glioblastoma. CD31 (red), FAM NPs (green), nuclei (blue).  Author: Maarja Haugas, PhD

Infiltrating CD206-positive macrophages in peritoneal MKN45P gastric carcinoma xenograft

Monday, 16 January 2017 11:07

Infiltrating CD206-positive macrophages in peritoneal MKN45P gastric carcinoma xenograft.
Author: Hedi Hunt, MSc.

Transmission electron microscopy image of a dextran coated iron oxide nanoworm structure

Friday, 16 December 2016 11:00

Red color was applied with photo editing. Merry Christmas!

Author: Allan Tobi, MSc

Glih27 peptide conjugated with iron oxide nanoworms (green) in mouse brain with VEGF KO glioblastoma

Wednesday, 16 November 2016 10:53

The nanoworms with peptide home to ependymal cells. Red – blood vessels, blue – DAPI.

Author: Pille Säälik, PhD   

Targeting-polymeric nanoparticles are internalized in MCF-7 and U87 spheroids

Monday, 31 October 2016 10:30

Fluorescein-labeled polymeric nanoparticles functionalized with a targeting peptide (in green) are internalized into MCF-7 (from breast tumor) and U87 (from glioblastoma) spheroids that express the NRP-1 receptor (in red). The nanoparticles are observed inside the U87 cells (white arrow). Scale bar are 200 µm and 20 µm (in the big magnification).

Authors: Lorena Simón-Gracia, Ines Diaz Bessone, Kadri Toome

An infiltratively growing patient-derived mouse glioma xenograft P21 developed in our lab expresses nestin (green) and Ki67 (red) positive cells.

Thursday, 15 September 2016 09:35

Cell nuclei are stained with DAPI (blue). 
Author: Pille Säälik, PhD

Intravenously injected "UNO" peptide enters​ protumoral macrophages

Monday, 15 August 2016 09:01

Angiogenic glioblastoma, WTGBM.

Microscope: Olympus FV1200MPE-BX61WI confocal microscope at 20X magnification Green: peptide, Red: tumor associated macrophages, blue: cell nuclei.

Authors: Pablo Scodeller PhD, Pille Säälik PhD

Nanoparticles homing to breast tumor

Monday, 18 July 2016 13:09

Left: electron microscopy images of polymeric nanoparticles targeted with tumor penetrating peptide loaded with cancer drug. Right: Confocal microscopy pictures of tumor, kidney, and liver tissue sections of mouse bearing breast tumor i.v. injected with nanoparticles loaded with cancer drug.

Authors: Ines Diaz Bessone PhD, Pablo David Scodeller PhD, Lorena Simon Gracia PhD

Two-photon image of blood vessels with Evans Blue after tail vein injection

Wednesday, 15 June 2016 12:51

Detection with multiphoton microscope Olympus FV1200MPE-BX61WI and Spectra-Physics MaiTai DeepSee IR laser. Objective: XLPLN25xW-MP (NA 1.05; Olympus). Excitation: 950nm. Author: Maarja Haugas, PhD

Hollow gold-silver nanospheres

Wednesday, 18 May 2016 09:33

Hollow gold-silver nanospheres imaged with a TEM at 160 000x magnification.

Author: Anne-Mari Anton Willmore, MSc

The "UNO" peptide recognizes​ circulating​ tumor​ associated​​ macrophages

Tuesday, 19 April 2016 09:21

Mouse model of metastatic human gastric cancer, MKN45-P.

Microscope: Olympus FV1200MPE-BX61WI confocal microscope at 20X magnification Green: peptide, Red: tumor associated macrophages, blue: cell nuclei.

Author: Pablo Scodeller, PhD

TT1-FAM-IONW

Wednesday, 23 March 2016 14:48

WT GBM intracranial glioma-bearing mouse was injected with 7,5 mg /kg TT1-FAM-iron oxide nanoworms. After 5 hours of circulation the mouse was perfused and FAM fluorescence was detected by illumatool. Accumulation of particles can be detected in the tumor area.

Author: Pille Säälik, PhD

A peptide identified in the lab using in vivo phage display homes to tumor macrophages in a mouse model of a highly metastatic human gastric cancer, MKN45-P

Wednesday, 17 February 2016 13:38

Microscope: Olympus FV1200MPE-BX61WI confocal microscope at 60X magnification

Green: peptide, Red: macrophages, blue: cell nuclei.

Author: Pablo David Scodeller, PhD

IONW cycle of life

Friday, 15 January 2016 15:17

Microscope: SEM (magnification: 600,000x).

Author: Allan Tobi, MSc student

Expression of stem cell markers Aldh1a1 and Nestin in human stem cell like glioblastoma cell line P3

Tuesday, 01 December 2015 14:06

Confocal detection with Olympus FV1200MPE-BX61WI microscope and 60xoil objective.
Author: Maarja Haugas, PhD

Ex vivo visualization of targeted nanoworms homing to intraperitoneal and subcutaneous tumors after i.v. injection

Monday, 09 November 2015 16:03

Mouse model: peritoneal gastric carcinoma ( MKN-45 in Nude mice).
Author: Hedi Hunt, MSc

Neuropilin 1 expression (in red) in glioblastoma cell lines used in our lab

Tuesday, 27 October 2015 13:34

WT GBM, VEGF KO GBM and 005 – mouse-derived; P3 and NCH421K – human-derived cell lines. Blue – nuclei (DAPI).
Image acqusition: confocal system Olympus FV1200MPE.
Author: Pille Säälik, PhD

Second harmonic generation image of collagen fibers in mouse dermis

Saturday, 26 September 2015 14:55

Detection in wavelength of 800nm with Spectra Physics MaiTai Deep See Ti:Sapphire laser. Multiphoton microscope Olympus FV1200MPE; objective XLPLN 25xW-MP (NA 1.05).
Author: Maarja Haugas, PhD

Targeted polymersomes penetrate deep into peritoneal tumors

Sunday, 23 August 2015 14:26

Targeted polymersomes (in green) target Neuropilin1 rich regions (in red) in gastric peritoneal tumors when injected intraperitoneally.
Authors: Lorena Simon Gracia, PhD and Pablo Scodeller, PhD

Homing of nanoworm nanoparticles to peritoneal gastric carcinoma ( MKN-45 Nude mice)

Tuesday, 07 July 2015 16:28

Sample collected 5h after i.p. injection. 
Red: Vessels
Green: NW-TT1-FAM
Blue: Nuclei
Technology: Confocal Microscopy (Olympus FV1200MPE)
Author: Hedi Hunt, MSc

Homing of polymersome nanoparticles to peritoneal colon carcinoma (CT26 Balb/c syngeneic tumor model, sample collected 24 hours after injection)

Thursday, 11 June 2015 15:33

Technology: confocal microscopy
Authors: Lorena Simon Gracia, PhD and Hedi Hunt, MSc

Ali Baba and fourty thieves a.k.a 41 T7 bacteriophages amplifying on the E. coli agar plate.

Friday, 15 May 2015 16:53

Author: Tarmo Mölder, MSc

U87 cell line spheroids taking up RPARPAR-AgNPs (red)

Wednesday, 15 April 2015 14:28

Technology: Confocal microscopy 20x
Author: Kadri Toome, MSc

In vivo imaging of a mouse bearing luciferase-expressing MKN-45 gastric carcinoma.

Thursday, 26 March 2015 14:25

Left: luminiscence of the gastric tumor. Right: fluorescence of polymeric vesicles homing to the tumor.
Images were acquired using ART OPTIX MX3 imaging system.
Authors: Lorena Simón Gracia, PhD and Pablo Scodeller, PhD

SKOV3 ovarian cancer cells

Wednesday, 18 February 2015 02:00

Technical information: PS-Rho-iRGD-FAM
Polymersomes-Red
iRGD peptide -Green
NRP-1-Purple
Nuclei-Blue
Author: Hedi Hunt, MSc

Co-culture of NRP1 (green) expressing PPC1 cells along with M21 cells; both cell lines express p32 (white), RPARPAR-AgNPs (red) are taken up by PPC1 cells.

Tuesday, 13 January 2015 02:00

Technical information: Confocal microscopy 63x.

Author: Anne-Mari Anton Willmore, Lorena Simon Gracia

PC3, prostate cancer cells, taking up RPARPAR-AgNPs (red), also showing NRP1 expression (green) and p32 expression (white)

Tuesday, 02 December 2014 15:44

Technical information: Confocal microscopy, 63x magnification.
Author: Anne-Mari Anton Willmore, MSc

Selective internalization of RPARPAR silver nanoparticles (Ag-RPARPAR) in NRP-1 expressing cells. In mixed culture, Ag-RPARPAR are taken by NRP-1 (green)-expressing PPC1 prostate carcinoma cells but not by M21 melanoma cells not expressing NRP-1.

Wednesday, 12 November 2014 08:34

Technical information: Confocal microscopy, 63x magnification.
Authors: Lorena Simon Gracia, PhD and Anne-Mari Anton Willmore, MSc

Confocal microscopy picture showing the internalization of polymersomes labeled with Rhodamine and targeted with RPARPAR-FAM peptide in PPC-1 cells. 63 x magnification.

Monday, 29 September 2014 13:06

Authors: Lorena Simon Garcia, PhD and Hedi Hunt, MSc

Transmission electron microscope image of hybrid silver/polymeric nanoparticles (NPs)

Friday, 29 August 2014 15:25

Ag-PVP NPs are encapsulated inside POEGMA-PDPA polymersomes. pH-sensitive polymersomes release the AgNPs and encapsulated drug inside the tumor cells. Targeted hybrid NPs can be used as a theranostic tool.
Author: Lorena Simón Gracia, PhD

Penetration of uCendR-RGD peptide to 4T1 breast tumors in vivo

Friday, 13 June 2014 10:07

uCendR peptide was designed by positioning the cryptic CendR motif  in the context of recognition and cleavage site of a tumor-associated protease, urokinase-type plasminogen activator (uPA).  FAM-labeled uCendR peptide containing additional RGD vascular recruitment module was  injected into  4T1breast tumor mice, after 3hr circulation the mice were perfused with PBS and tumors sectioned for microscopy. Confocal microscopy confirmed the accumulation of synthetic uCendR-RGD peptide (green) in tumor tissue, and revealed extravasation and spreading within the tumor. CD31- positive tumor blood vessels (bv) – red.
Author: Tambet Teesalu, PhD

Intravenously injected phage displaying a candidate glioma homing peptide (green) colocalizes with CD68-positive glioma macrophages (red), nuclei-blue.

Monday, 16 December 2013 16:32

Model: VEGF KO GBM, collaboration with Gabriele Bergers (UCSF).
Authors: Kadri Toome, MSc and Pille Säälik, PhD

109Ag nanoparticles as seen with TEM 210k X and coupled with fluorescent dye and CendR peptide being taken up by cancer cells 100 X

Wednesday, 16 October 2013 13:37

Author: Anne-Mari Willmore, MSc

TEM picture of 20 nm silver nanoparticles at 65,000x of magnification

Wednesday, 08 May 2013 14:20

Author: Lorena Simon Gracia, PhD

TEM image of silver, palladium nanoparticles at 93,000x magnification

Saturday, 20 April 2013 11:56

Author: Anne-Mari Willmore, MSc

Internalization of prototypic CendR (RPARPAR) phage by PPC1 prostate carcinoma cells in culture

Saturday, 15 December 2012 02:00

Phage – green, phalloidin – red, DAPI-blue
Aithor: Tambet Teesalu, PhD

TEM image of AgPVP nanoparticles at 73,000x magnification

Thursday, 15 November 2012 02:00

Author: Lorena Simón Gracia, PhD

TEM image of PEG-PDPA Polymersomes at 105,000x magnification

Monday, 15 October 2012 03:00

Author: Lorena Simón Gracia, PhD

AgPVP nanoparticles at 105,000x magnification

Saturday, 15 September 2012 03:00

Author: Anne-Mari Willmore, MSc